Culturing Saltwater Phytoplankton Guide

Culture Phytoplankton (Marine Algae) using PHYTO - FOOD (Algae Nutrient).

Nannochloropsis, Tetraselmis, Isochrysis, and other marine saltwater micro algae.

Start with a culture container (1 or 2 Liter Bottle). Fill with New mixed saltwater at between 1.020 – 1.025 salinity. Can use old aquarium water but must sterilize by boiling.

Add 1 ml Phyto- Food Per 2 liter bottle. This will provide enough nutrients for algae to mature and consume for 5 – 7 days. Period will depend on Photo period. (length of hours of light and dark)

Add your algae starter culture to this concoction.

 Water  temperature kept between 72- 85 Deg. F

Add an Air stone with medium aeration to help continuously  mix algae cells. This is to prevent algae from gathering in one place and blocking light penetration.

Can run light photo period 24 hours.  Any wattage light bulbs will do. The higher wattage the better.  Sunlight from a window will also do. Cheap 40 watt florescent bulbs and fixtures work great. Try to place bulbs as close to bottles as possible.

Wait and harvest after 5 -7 days.

Example: If you want to harvest your Isochrysis algae culture on a daily basis. Wait until your culture is nice and thick. Then start one culture every day for 5 days. Staggering each successive batch one day apart will yield one batch for use per day.